HPLC法测定姜状三七根茎中的人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1
Determination of notoginsenoside R1、ginsenoside Rg1 andginsenoside Rb1 in Panax zingiberensis by HPLC
-
摘要: 建立人参属植物姜状三七根茎中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量测定方法.采用HPLC法,色谱柱为Phenomenex C18(250 mm× 4.6 mm,5 μm);流速为1.0 mL/min;检测波长203 mm;柱温30 ℃;流动相为乙腈-水(梯度洗脱).三七皂苷R1的线性范围为0.315~1.575 μg(r= 0.999 1),平均回收率为三七皂苷R1101.4%,RSD=1.79%;人参皂苷Rg1的线性范围为1.203~6.015 μg(r=0.999 8),平均回收率为人参皂苷Rg198.54%,RSD=1.90%;人参皂苷Rb1的线性范围为0.276~1.38 μg(r=0.999 6),平均回收率人参皂苷Rb1102.10%,RSD=1.53%.所建方法简便、准确、重复性好,可同时测定姜状三七根茎中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量.Abstract: A method is established for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.A HPLC method was adopted.The analysis was carried out on all analytical column C18(50 mm×4.6 mm,luna 5 μm).The flow rate was 1.0 mL·min-1 and the detective wavelength was set at 203 mm with column temperature of 30 ℃.The mobile phase consisted of acetonitrile-water(gradient elution).The linear range was 0.315—1.575 μg(r=0.999 1)and the average recovery was 101.4％with the RSD of 1.79％ for notoginsenoside R1.The linear range was 1.203—6.015 μg(r=0.999 8)and the average recovery was 98.54％with the RSD of 1.9％ for ginsenoside Rg1.The linear range was 0.276—1.38 μg(r= 0.999 6)and the average recovery was 102.1％with the RSD of 1.53％ for ginsenoside Rb1.The method is simple,reproducible and suitable for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.
下载: