Abstract: To study on chemical constituents of Daphne aurantiaca Diels.Eight known compounds including four flavones and four coumarins were isolated by medium-pressure column chromatography and preparative HPLC.Their structures were elucidated as daphnin(1),daphnetin(2),orientin(3),isoorientin(4),quercetin(5),genkwanin(6),daphnoretin(7)and acetylumbelliferone(8)by physicochemical properties and spectral analysis.All compounds were isolated from this plant for the first time.Their in vitro cytotoxic activities were evaluated against two prostate cancer cell lines PC-3M and LNCaP,showing that daphnoretin(7)was the most potential agent with IC50 values of 8.1 and 13.5g/mL,respectively.