Abstract: In order to protect and develop the wild rattan resources in Yunnan,to improve the technology of artificial cultivation,the seeds of Calamus gracilis which had been steeped in different concentrations of biological hormone GA (20、50、100mg/L),and pure water,were carry out the breeding experiment in greenhouse of Southwest Forestry University.A hotbed had been laid under the seedling bed and a small greenhouse was built.The results showed that: the thousand-grain weight with shell was 9044.36 g,without shell was 4029.07 g,the hard peel and thick flesh might be one of the reasons for the low germination rate of the seeds under the natural environment.The small greenhouse and hotbed provided a good condition for the seed germination which was similar to the water and heat condition in Xishuangbanna area,with average temperature at 27.6 degree,average relative humidity at 89%,and average soil temperature at 25.4 degree.By analyzing the indices of seed germination rate,root diameter,root length,root diameter and seedling height,the seeds treated by GA (A3 100mg/L) had the highest germination rate (88.04%),and the best growth rate.After transplanting,the seedlings growth by different treatments had no significant difference (P>0.05).Therefore,it is important to improve the water and heat condition during the seedling germination and the seed germination rate can be improved by biological hormone treatment.