Abstract: Pyrazino-bis-steroids are a group of complex steroid-alkaloid hybrids.These compounds not only possess the unique structural characteristics,but also show potent antitumor biological activities.They are difficult to obtain from the natural source and it has limited their bioactive studies.In this paper,a facile while flexible method towards the symmetric synthesis of the pyrazino-bis-steroids was developed using steroids such as hecogenin,choesterol,ergosterol,srigmaterol as basic materials.Pyrazino-bis-steroids were achieved in only one step by reacting of the key intermediate, 2-hydroxy-or 4-hydroxy-3-one steroid derivatives with ammonium acetate.Ten pyrazino-bis-steroids compounds were synthesized and seven of which were reported first time by our methodology.More symmetric pyrazino-bis-steroids should be easily synthesized for further studies easily with our methodology compared with the literature methods.