Abstract: Oatmeal β-glucan has nutritional and health effects.In order to explore and utilize the genotype and germplasm resources of β-glucan in oat kernel,wild or cultivated oats from Italy (31)and Shanxi (25) were used as research materials.The content of β-glucan in the oat kernel was determined and analyzed in combination with its nutritional traits.Analysis was performed by principal component analysis (PCA) and correlation network analysis (CAN).The results showed that the content of β-glucan was significantly correlated with total flavonoids and amino acids,and was not correlated with other nutritional traits.The content of β-glucan was significantly different between different groups and different genotypes.The content of β-glucan in 56 oats was between 1.76% and 8.72%.The genotypes of ITAO1 and XO-1-13 with high content of β-glucan in oat kernel were screened out.