Abstract: Objective To study the rapid and sensitive method for the determination of veratramine in human plasma by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) and provide a stable and reliable method for the determination of veratramine blood concentration. Methods The plasma sample was extracted with ethyl acetate and phenacetin as internal standard.After the sample was processed,acetonitrile-10 mmol·L-1 ammonium acetate (pH adjusted to 8.8) was used as mobile phase.In positive ion mode,multiple reaction monitoring was used ( MRM) quantification,veratramine quantification ion pair m/z:410.1/295.1,phenacetin quantification ion pair m/z:180.2→110.0. Results Veratramine in 0.201—6.45ng/mL linear range,three consecutive batches of standard curves correlation coefficient r are greater than 0.99 (weight 1/X2),lower limit of quantitation was 0.201 ng/mL;high,medium,low three concentrations day precision (RSD%)≤15.11%,accuracy ranged between 96.52%—102.42%;the matrix does not affect the measurement effect. Conclusion The established LC-MS/MS method is rapid,sensitive,specific,and highly reproducible.It is suitable for determination of human plasma concentrations of veratramine.