Abstract:
In this study, 18 kinds of imprinted polymers (MIP
1~18) and corresponding non-imprinted polymers (NIP
1~18) were prepared by using
S-2-mercaptopropionic acid as chiral functional monomer,
S-naproxen (
S-Npx) as template molecule. Experimental results show that the optimal imprinted polymers were prepared by nano-silica (Nano-SiO
2) as carrier, surface imprinting technique, the molar ratio of template molecule, functional monomer and crosslinker was 1∶4∶20, and methanol was used as porogen solvent. The adsorption behavior was studied by Langmuir and Freundlich adsorption isotherm models. The results show that the adsorption process was mainly based on multimolecular layer adsorption. Scatchard analysis shows that the maximum apparent binding capacity
Qmax of the dissociation constant
Kd was 0.82 mmol/L,
Qmax was 9.92 mg/g. The kinetic study shows that when the adsorption equilibrium is reached, the maximum adsorption capacity can reach 5.73 mg/g, imprinting factor of 4.82, and the adsorption process accords with the quasi-second-order kinetic model. The structure of the optimal polymer MIP
3 and the corresponding NIP
3 was characterized by the scanning electron microscope (SEM). Also, the chiral resolution of MIP
3 racemic
R,
S-naproxen (
R,
S-Npx) was studied. Finally, MIP
3 was studied with HPLC, which reflected the mixed naproxen was separated successfully. The resolution factor reached 2.31 which proved that the resolution ability was well.