Abstract: The gene for CaM and CaMBp was transferred into tobacco leaf disks with help of Ti plasmid of Agrobacterium tumefaciens. Tobacco leaf disks were rooted in MS medium containing hygromycin after pre culture,agroinfection and co culture in 2～3 months.We established system of trangenic tobacco which will express calmodulin or calmodulin binding protein.In this experiment, it was found there were many effect foctors.The effect of agroinfection on plant in different growth period was different and time of transgene were affected by different lines. The culture and dilution of Agrobacterium tumefaciens influenced trangenic efficiency.The co culture of plant and Agrobacterium tumefaciens aslo influenced trangenic efficiency.It was important for root of seedling to be screened that could select out transgene tobacco.