Abstract: The chemical constituents of Pittosporum podocarpum Gagnep were studied by methods of extraction,column chromatography,crystallization and so on.Nine compounds were isolated from this plant by column chromatogramphies of sephadex LH-20 and silica gel.Their structures were identified by analyses of their physical-chemical properties and spectral datas.All these compounds were isolated for the first time from this plant.These are:kielcorin(1); 2,3-dimethoxyxanthone(2); quercitrin-3,7–dimethoxy(3); luteolin(4); lupeol(5); cerotic acid(6); 1-octacosanic acid(7);β-sitosterol(8); daucosterol(9).Cytotoxicity screening of extracts from Pittosporum podocarpum Gagnep was carried out by HL60 cell.Three extracting fractions of petroleum ether,chloroforn and ethyl acetate possess cytotoxic activities and the IC50 are 6.5,3.5 and 5.0 μg/mL for HL60,respectively.