12个草地早熟禾品种愈伤组织诱导体系的研究
A study on the callus induction system in 12 Poa pratensis L.varieties
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摘要: 以生产中常见的12种草地早熟禾(Poa pratensis L.)成熟种子为外植体,对其愈伤组织诱导及愈伤组织增殖生长体系进行了优化试验.结果表明,在愈伤组织诱导试验中,单纯使用2,4-D时,不同品种愈伤组织诱导率在60%~93%之间,2,4-D质量浓度3 mg/L时12个早熟禾品种的愈伤组织诱导率均值最高,达到86%;在2,4-D基础上添加少量6-BA(0.1~0.2 mg/L)后,不同品种愈伤组织诱导率在30%~92%之间,12个品种的愈伤组织诱导率均值有所降低(3.1%~25.4%).在愈伤组织增殖试验中,单纯使用2,4-D时,不同品种愈伤组织增殖率在38%~164%之间,12个早熟禾品种的愈伤组织增殖率均值在3 mg/L 2,4-D时最高,达到81%;在3 mg/L 2,4-D基础上施加少量的6-BA(0.05~0.2 mg/L)后,愈伤组织增殖率均值有所降低,但存在品种的差异性.研究结果还显示,热激处理对部分草地早熟禾愈伤组织诱导及增殖有一定的促进作用.Abstract: 12 Kentucky bluegrass varieties (Poa pratensis L.) were randomly selected as the materials for the test of callus induction and proliferation,taking mature seeds as explants.Varieties showed different rates in forming callus.With 2,4-D only,it varied from 60% to 93%.With 2,4-D at 3mg/L,the average rate of 12 varieties reached to 86%.By adding some 6-BA(0.1-0.2 mg/L)additionally,the rates changed from 30% to 92%,but the average rate was reduced a little.In the callus proliferation test,the proliferation rates were in a range of 38%-164% with only 2,4-D.The average rate reaches to 81% with the treatment of 3 mg/L 2,4-D.Additional 6-BA at 0.05-0.2 mg/L reduced the average proliferation rate,and the reduction was changing by different varieties.The results also showed that the heat shock increased the rate of callus induction and proliferation for some Kentucky bluegrass varieties.
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