Abstract: Molecular recognition behavior of bisphenol A by β-cyclodextrin was studied with ultraviolet spectrum,infrared spectrum,fluorescence spectrum and 1HNMR,the results from ultraviolet spectrum showed that the absorbance of bisphenol A after molecular recognition increases with increasing of the concentration of β-cyclodextrin and results in 2—3 nm red shift of maximum absorbance wavelength,the results from infrared spectrum showed that some characteristic peaks of bisphenol A after molecular recognition were covered and results in shift of absorbance peaks,the results from fluorescence spectrum showed that the fluorescence intensity of bisphenol A after molecular recognition increases with increasing of the concentration of β-cyclodextrin.β-cyclodextrin reacts with bisphenol A forming a 1:1 inclusion complex,the formation constant of which is 1.84×104 L·mol-1,bisphenol A can enter the inner cavity of β-CD from the bigger opening.