Abstract: By using Taxus yunnanensis as experiment material,different methods including paraffin embed,squashed with aceto-camine and stain-clearing,methods of sporogenesis,paraffin embedded samples and cultured in vitro methods for microgametogenesis were used and evaluated.In cultured in vitro methods,the influence of culture temperature,the sucrose concentration of medium and staining methods on the results was studied.The results can be summarized as follows:stain-clearing technique is a better method for sporogenesis due to its simple operation and better results.Raffin embedded samples and cultured in vitro are necessary methods in gametogenesis,and in vitro,sterilizing on surface and releasing microspores by itself,low temperature and low sucrose concentration are helpful to eliminate contamination.It is convenient that male gametogenesis in vitro be stained by aceto-carmine.