Abstract: Fifty rice inflorescence related genes from Internet,references were chosen.After probes of the 50 genes were designed and screened by BLAST analysis in the Internet,they were synthesized,purified and micro-arrayed onto the microscope sliders.Total RNA from 3 kinds of rice inflorescence tissues was extracted and labeled by fluorescent dye.The labeled probes were then hybridized with the oligonucleotide arrays.Expression patterns of 50 genes from rice inflorescence in different development were obtained by detection using ScanArray3000.It showed analyzed pots with uniformity background and clear signal.The plots of the images were acquired after the acquired gene expression patterns were analyzed by ImaGene4.0 software.The scatter plots and scale maps show that there existed a significant difference in the expression of these candidate genes in rice inflorescences with different development phase.Then,a foundation for the preparation of rice oligonucleotide microarray and farther application was layed.