Abstract:
In order to explore the feasibility of culturing influenza virus on KMB17 cells and optimize the culture condition,four strains of influenza virus were inoculated on KMB17 cells,they were cultured in serum free medium under different pH condition and different trypsin concentration.Then,they were been harvested after 72 hours,and the HA titers were detected.The HA titers of the H3N2 subtype strain are significantly higher than the other two stains(one is H1N1 subtype and the other is B type).It was concluded that,under some condition,KMB17 cells can be an alternative medium for influenza viruses cultivation.