Abstract: Starting from Coenzyme Q0,backbone of Q10;1,4-dimethoxymethylether-2,3-dimethoxy-5-bromotoluene was synthesized.Coenzyme Q0 was reduced to give hydroquinone which was bromized,then the phenol group of hydroquinone was protected with chloromethyl mehthyl ether.Then Coenzyme Q10 was synthesized in high yield by the coupling reaction between Grignard reagent of 1,4-dimethoxymethylether-2,3-dimethoxy-5-bromotoluene and decaprenyl bromide catalysted by CuI,followed by deprotection and oxidation of the corresponding toluene derivative.It is the frist time to synthesize Coenzyme Q10 by the coupling reaction between Grignard reagent of backbone of Q10 and decaprenyl bromide.