Abstract: Bacillus paramycoides plays a significant role in processes such as high-efficiency degradation of pesticides, feather decomposition, and hospital wastewater treatment. In this study, a strain with good flocculation performance was screened from water samples taken from Siyuan Lake at Guangxi University for Nationalities. Through 16S rRNA gene sequencing and phylogenetic tree analysis, this strain was identified as Bacillus paramycoides, designated as GXUN74708. Physiological and biochemical tests, as well as electron microscopy scans, revealed that strain Bacillus paramycoides GXUN74708 is a Gram-positive bacterium, rod-shaped, motile, capable of synthesizing catalase and protease. By optimizing single-factor control conditions such as initial inoculum size, fermentation time, initial pH of the medium, and amount of fermentation broth added, it was found that the flocculation rate of strain Bacillus paramycoides GXUN74708 could reach up to 99.1%. Furthermore, it was discovered that the microbial flocculant synthesized and secreted by strain Bacillus paramycoides GXUN74708 is mainly distributed in the supernatant of the fermentation broth. The strain identified in this study, Bacillus paramycoides GXUN74708, exhibited excellent flocculation performance in wastewater treatment. The composition of the medium used for fermentation by the strain is simple, and under conditions of 30°C, 180 r/min, and shaking bed fermentation for 36 hours, fermentation broth with good flocculation performance can be obtained. Therefore, it is evident that strain Bacillus paramycoides GXUN74708 has simple and non-stringent fermentation conditions, indicating its potential application value in wastewater treatment.