Abstract: Restriction-site associated DNA sequencing (RAD-seq) is a reduced-representation sequencing technology developed from next generation sequencing technology.The technology is simple to operate,experimental low cost and greatly simplify the genomic complexity.Fish is the group with the largest number of species in vertebrates and has rich species diversity due to different living conditions,which is often used as research subjects in genomic studies.At present,RAD-seq technology has been widely used in phylogenetic relationships,species differentiation,genetic map,population genetics and adaptive evolution for fish genomics research.The research progress of RAD-seq in fish genomics is summarized in this paper and reference evidence is thus provided for conservation and rational use of fish resources.