RAD-seq技术在鱼类基因组学中的研究进展

刘涛 李蓉 肖蘅 陈善元

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RAD-seq技术在鱼类基因组学中的研究进展

    作者简介: 刘涛(1992-),男,云南人,硕士生,主要从事鱼类分子遗传与进化研究.E-mail:745018389@qq.com;李蓉(1990-),女,云南人,博士生,主要从事动物遗传学研究.E-mail:12014000884@mail.ynu.edu.cn.刘涛和李蓉对本文具有同等贡献.;刘涛(1992-),男,云南人,硕士生,主要从事鱼类分子遗传与进化研究.E-mail:745018389@qq.com;李蓉(1990-),女,云南人,博士生,主要从事动物遗传学研究.E-mail:12014000884@mail.ynu.edu.cn.刘涛和李蓉对本文具有同等贡献.;
    通讯作者: 肖蘅, xiaoheng@ynu.edu.cn ; 陈善元, xiaoheng@ynu.edu.cn
  • 基金项目:

    国家自然科学基金地区科学基金(31560111)

    云南省“百名海外高层次人才引进计划”项目(W8110305).

Research progress of RAD-seq in fish genomics

  • 摘要: 限制性酶切位点相关DNA测序(restriction-site associated DNA sequencing,RAD-seq)是在二代测序技术上发展起来的简化基因组测序技术,该技术操作简单、实验成本低、并且简化了基因组的复杂度.鱼类是脊椎动物中物种数最多的类群,由于生活环境的不同,造就了丰富的物种多样性,常作为基因组学的研究对象.目前,在鱼类基因组学研究中,RAD-seq技术广泛应用于鱼类的系统发育、物种分化、遗传图谱、群体遗传和适应性进化等方面的研究.研究主要基于RAD-seq技术在鱼类基因组学中的研究进展进行综述,以期为鱼类资源的保护和合理利用提供参考依据.
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RAD-seq技术在鱼类基因组学中的研究进展

    作者简介:刘涛(1992-),男,云南人,硕士生,主要从事鱼类分子遗传与进化研究.E-mail:745018389@qq.com;李蓉(1990-),女,云南人,博士生,主要从事动物遗传学研究.E-mail:12014000884@mail.ynu.edu.cn.刘涛和李蓉对本文具有同等贡献.
    作者简介:刘涛(1992-),男,云南人,硕士生,主要从事鱼类分子遗传与进化研究.E-mail:745018389@qq.com;李蓉(1990-),女,云南人,博士生,主要从事动物遗传学研究.E-mail:12014000884@mail.ynu.edu.cn.刘涛和李蓉对本文具有同等贡献.
    通讯作者: 肖蘅, xiaoheng@ynu.edu.cn
    通讯作者: 陈善元, xiaoheng@ynu.edu.cn
  • 1. 云南大学 生命科学学院,云南 昆明 650091
基金项目:  国家自然科学基金地区科学基金(31560111) 云南省“百名海外高层次人才引进计划”项目(W8110305).

摘要: 限制性酶切位点相关DNA测序(restriction-site associated DNA sequencing,RAD-seq)是在二代测序技术上发展起来的简化基因组测序技术,该技术操作简单、实验成本低、并且简化了基因组的复杂度.鱼类是脊椎动物中物种数最多的类群,由于生活环境的不同,造就了丰富的物种多样性,常作为基因组学的研究对象.目前,在鱼类基因组学研究中,RAD-seq技术广泛应用于鱼类的系统发育、物种分化、遗传图谱、群体遗传和适应性进化等方面的研究.研究主要基于RAD-seq技术在鱼类基因组学中的研究进展进行综述,以期为鱼类资源的保护和合理利用提供参考依据.

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